One of the most dominant competitive performances ever witnessed on TV is over — James Holzhauer has been defeated, his remarkable run on “Jeopardy!” stopped just short of the iconic gameshow’s all-time winnings record.

Emma Boettcher, a user experience librarian from Chicago, won $46,801 to defeat Holzhauer on Monday’s episode, ending his prolific 32-game winning streak that captivated the nation.

In 33 games, Holzhauer won $2,464,216. He was poised to take over the top spot in the show’s all-time, regular-play winnings list with one more big win, but he’ll settle for second, $56,484 behind computer scientist and author Ken Jennings, who won $2,520,700 during an epic 74-game winning streak in 2004.

On Monday’s show, Boettcher grabbed the lead in the Double Jeopardy round and put the champ away with a $20,000 wager and the correct response of “Who is Christopher Marlowe?” in Final Jeopardy.

“Oh, gosh. What a payday,” host Alex Trebek exclaimed, as a smiling Holzhauer walked across the stage to give Boettcher a congratulatory high-five.

Boettcher was quick on the buzzer and took a slim lead into Final Jeopardy with $26,600. Holzhauer had $23,400, and Jay Sexton, in third place, had $11,000.

The category was “Shakespeare’s Time.” The clue: “The line ‘a great reckonings in a little room’ in ‘As You Like It’ is usually taken to refer to this author’s premature dream.” All three contestants provided the correct response of Christopher Marlowe, an English playwright from the 1500s.

Holzhauer wagered $1,399, enough to win if Boettcher misfired and hold off Sexton if he doubled up. Boettcher wagered $20,000 to win comfortably. Even if Holzhauer had wagered it all, he would have lost by $1.

“I was a little shook during Double Jeopardy,” Holzhauer wrote in an email, “because I was playing from behind and constantly getting beaten on the buzzer by Emma. By the time Final Jeopardy rolled around, I knew I could only win if Emma answered incorrectly. It felt like needing a team to miss a last-second field goal. She didn’t miss, but I was still proud of my performance the whole way. I gave her a high five and smiled at how far I’d come.”

Boettcher could not immediately be reached for comment and representatives from “Jeopardy!” did not respond to emails and calls from ESPN.

“I’m a person who really hates losing,” Holzhauer added, “but I learned that I’m proud of my place in the show’s history, even if it’s not at the absolute top of the pyramid.”

Though he came up shy of Jennings’ winnings record, Holzhauer’s mark on the game won’t soon be forgotten. In fact, many “Jeopardy!” diehards believe he changed how the game will be played going forward.

Holzhauer’s episodes were taped beginning in February. His run began airing on TV on April 4. Over the next three months, the charismatic, smiley 34-year-old professional sports bettor from Las Vegas exploded into mainstream pop-culture with an all-in, high-stakes gambling approach to “Jeopardy!”

He rarely missed — including Monday when he went 25 for 25 on answers 00 and routinely wagered large amounts on the 76 Daily Doubles that he found. He finished with 1,186 correct answers and only 36 incorrect. His average margin of victory was $64,903.

When it was over, he had authored the 16 most-prolific performances in the show’s 35 seasons, and 23 of top 27 overall. On April 9’s show, his fourth episode, Holzhauer shattered the previous single-game winnings mark with $110,914, besting the previous mark of $77,000 set in 2010 by Roger Craig.

Holzhauer eclipsed his own record twice, highlighted by a $131,127 performance on April 17, and repeatedly put away his competitors before Final Jeopardy. Only three times in 32 shows was a competitor in striking distance entering the final round. Monday’s episode was a different story, though

Boettcher didn’t miss, either, correctly responding to 22 clues. She went head-to-head with Holzhauer on the buzzer, too. According to thejeopardyfan.com, Holzhauer buzzed in first on 42.11 percent of clues, his worst performance since his second game on April. 5.

His incredible dominance caught the attention of the sports world. New England Patriots quarterback Tom Brady tweeted about “Jeopardy James,” and executives for Major League Baseball franchised publicly expressed interest in his skills. Holzhauer became a national sensation and watched it all play out on TV. He was bound by a standard non-disclosure agreement to keep the final result a secret, though.

Last week at Caesars Palace, while speaking at a gaming conference hosted by the UNLV, Holzhauer joked with a member of the audience from Canada, asking about any potential tax breaks for winnings if he were to move north.

Throughout his appearances, Holzhauer had colorful back-and-forth banter with Trebek about everything from sports betting to getting married in his bathing suit.

“[Trebek] always shakes the champ’s hand first [after the show], and it was weird when he didn’t head straight for me,” Holzhauer added. “Back in the green room, one of the producers called out “Champ!” to get Emma’s attention, and I reflexively turned my head to respond. That was when it really hit me that I’m not “champ” anymore.”

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