Joe Mixon appeared in an Ohio courtroom on Wednesday to fight back in his aggravated menacing case … pleading not guilty to the misdemeanor charge he was hit with earlier this month.

The NFL star wore a white button-down shirt and dark pants for the occasion … and said nothing to reporters neither during his arrival nor during his exit at his arraignment hearing in Hamilton County.

During the proceedings, Mixon entered his plea … and his bond was set at $10,000.

As we reported, Mixon was initially charged with aggravated menacing on Feb. 2 — after prosecutors said he threatened to shoot a woman during a road rage incident on Jan. 21.

The charge was dismissed after officials said they needed “additional investigation” — but it was refiled on April 7 after authorities said they found new evidence in the case.

The Bengals said in a statement at the time of the refiling that they were aware of the charge and were “monitoring the situation.”

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